|
|
|
|
q Introduction
The CELLine
bioreactor is a disposable, two-compartment cultivation device suitable for
many cell culture applications, e. g. the production of monoclonal antibodies
on a laboratory scale. Two different sizes of the CELLine are available:
CELLine CL350 and CL 1000. Details are listed below.
Efficient cell
cultivation is dependent on an optimal supply of oxygen and nutrients, as well
as an efficient removal of inhibiting metabolic waste products. In static cell
culture the optimal balance of these factors is not given. These limitations
lead to a reduced cell growth and are unfavorable for achieving high levels of
protein expression.
The
two-compartment bioreactor CELLine is designed to overcome these limitations by
dividing the bioreactor into a medium compartment and a cell compartment. A
semi-permeable membrane (10 kDa MWCO) between the compartments allows small
molecules to diffuse from one compartment to the other. Higher molecular weight
molecules secreted by the proliferating cells are retained within the cell
compartment. This results in a continuous flow of nutrients into the cell
compartment and a concurrent removal of any inhibitory waste products. The cell
compartment and the medium compartment are individually accessible which
enables the cells to be supplied with fresh medium according to their
individual needs. In addition, the bottom of the cell compartment is made
of a gas permeable membrane which ensures an optimal oxygen supply to and
carbon dioxide exchange from the cell culture. All together, this bioreactor
construction allows optimal maintenance of the cells at quasi in vivo
cultivation conditions. These conditions enable cells to proliferate to
very high densities within the cell compartment.
The CELLine is
perfectly suited for a wide range of applications involving suspension cell
culture, like monoclonal antibody production or long-term continuous
culture maintenance. The unit is optimised for cultivation of hybridoma,
CHO (Chinese hamster ovary) cells, NSO (mouse myeloma) cells, SF (Spodoptera
frugiperda, insect cells) cells and many more.
q Features and Benefits
High cell density & high product concentration - 10 to 100
times higher cell densities (107 to 109 cells/ml) - up to 100 times
higher protein concentration - 30-150 mg mAb
per 2 weeks with 1 CELLine Reduced hands-on time - Disposable - Ready-to-use - CELLine
replaces up to 10 mice and 20 T-Flask Cost-efficient and Space saving -
pre-concentrated product, less efforts in down stream processing - no additional
equipment, like agitation or control units are necessary - running costs
are low due to low media consumption
q ÁÖ¹® ¾È³» |