|
|
|
|
VoluPAC
VoluPAC tubes Disposable spin
tubes for the determination of biomass in cell culture suspensions, expressed
as packed cell volume (PCV)
Maintaining cell
lines in culture, under optimal growth conditions, is essential for
Many labs still
rely on a method, which has already been used for decades: manual cell count
using the hemacytometer. This method is both time consuming and labor-intensive.
Observed variation in results can be significant among users due to the
subjective nature of the determination. In addition, only several hundred cells
are generally counted, which means statistical confidence is low.
Automated devices
for cell count and viability determination are available, which are based on
either digital image recognition, the electrical impedance principle or flow
cytometry, respectively. They offer a faster and more precise approach, but are
usually regarded as costly concepts and often require additional dilution steps
and frequent calibration.
The VoluPAC tubes
provide a fast, easy to use, reproducible and reliable alternative to manual
cell counting. They are 15 times faster and 5 times more accurate (margin of
error less than 5%).
The VoluPAC tubes
enable the determination of the packed cell volume (PCV) in a cell suspension,
resulting in absolute data correlating with parameters like protein content,
cell count, metabolic activity and others.
The VoluPAC tubes
are particularly convenient to work with, requiring neither special training
nor sophisticated expensive machines - in contrast to automated cell counting
devices.
- Fast- 1 min
centrifugation compared to 5-15 min counting under the microscope - Reliable- Less
prone to statistical errors due to the high number of cells in the pellet - Reproducible- No user to user variations; no dilutions, manipulations,
or calibrations are necessary - Convenient and easy to use- Even for inexperienced users, no elaborate training
necessary - Works for all cell lines- No problems with cells forming microclusters - Allows parallel determination of many samples- Manual counting has to be performed sequentially - Compatible with standard rotors- No additional equipment required except a bench top
centrifuge - Disposable- Does not afford preliminary cleaning steps - Cost effective- Compared to highly sophisticated techniques, particularly
under concern of initial investment costs and maintenance - Provides absolute data- Irrespective of the actual volume of the cell culture With these
features biomass determination with VoluPAC is one of the simplest and most
rapid methods for obtaining precise information about a cell culture. The
overall accuracy competes with the best currently available techniques.
q ÁÖ¹® ¾È³» |