VIVA DNA Polymerase

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  VIVA DNA Polymerases
  Vivagen Co., Ltd., Çѱ¹

q Á¦Ç° Á¾·ù ¹× ÁÖ¹® ¾È³»

Polymerase Á¾·ù

ÁÖ¹® ¹øÈ£

Unit

Á¦Ç°±¸¼º

Taq DNA Polymerase

T011

T012

500 U/250§¡

1,000 U/500§¡

Polymerase

1 tube

2.5 mM dNTP

1 ml

10X buffer (w/MgCl2)

2 ml

Gel loading buffer (6X)

1 ml

DNA Size Marker

50 §¡

Taq Plus DNA Polymerase

T021

T022

500 U/250§¡

1,000 U/500§¡

Polymerase

1 tube

2.5 mM dNTP

1 ml

10X buffer (w/MgCl2)

2 ml

Gel loading buffer (6X)

1 ml

DNA Size Marker

50 §¡

Pfu DNA Polymerase

T031

T032

500 U/250§¡

1,000 U/500§¡

Polymerase

1 tube

2.5 mM dNTP

1 ml

10X buffer (w/MgCl2)

2 ml

Gel loading buffer (6X)

1 ml

DNA Size Marker

50 §¡

 Hot-Taq DNA Polymerase

T041

T042

500 U/250§¡

1,000 U/500§¡

Polymerase

1 tube

2.5 mM dNTP

1 ml

10X buffer (w/MgCl2)

2 ml

Gel loading buffer (6X)

1 ml

DNA Size Marker

50 §¡

25mM dNTP mixture

(total 100mM)

T050

25/mM500§¡

 

 

2X Taq Master mix

TM011

500§¡ X 4 tubes

Taq premix

4 tubes

DNA MW Marker

50 §¡

Gel loading buffer (6X)

1 ml

2X Taq Master mix

TM012

96well

(8strip-tubes x 12ea)

Taq Plus premix

4 tubes

DNA MW Marker

50 §¡

Gel loading buffer (6X)

1 ml

 

* ¿¬±¸ÀÚ¿äû¿¡ µû¶ó MgCl2¾ø´Â ¹öÆÛÁ¦°øµµ °¡´ÉÇÕ´Ï´Ù.

 

q ¸Å´º¾ó

 

l   VIVA¢â Taq DNA Polymerase

l   VIVA¢â Taq Plus DNA Polymerase

l   VIVA¢â Pfu DNA Polymerase

l   VIVA¢â Hot-Taq DNA Polymerase

l   VIVA¢â 2X Taq Master mix

 

q Description

 

¢ßºñ¹ÙÁ¨¿¡¼­ Á¦Á¶µÈ PCR °ü·Ã DNA polymeraseµéÀº °í¼øµµ·Î Á¤Á¦µÈ enzymeÀ¸·Î¼­ ³ôÀº accuracy ¹× Çâ»óµÈ fidelity¸¦ Á¦°øÇϸç ƯÈ÷ ¾ö¼±µÈ stability test¸¦ ÅëÇØ ¿¬±¸ÀÚ·Î ÇÏ¿©±Ý ÃÖÀûÀÇ PCR reactionÀ» ¼öÇàÇϵµ·Ï µµ¿Í ÁÝ´Ï´Ù.

 

q Characterization

 

Polymerase

Max product size(kb)/

E. coli genomic DNA

Proofreading

(3¡¯ to 5¡¯ exo)

Specificity

Product end

Application

Taq

10

Yes

+++

3¡¯-A

ÀϹÝÀûÀÎ PCR ¿ëµµ·Î ÀûÇÕ.

Taq Plus

15

Yes

++++

3¡¯-A

±æÀÌ°¡ ±ä DNAÀÇ ÁõÆø ½Ã »ç¿ë.

Hot-Taq

5

No

++++

3¡¯-A

Hot start PCR¿¡ ÀûÇÕ, ³ôÀº ¹Î°¨µµ

Pfu

10

Yes

+++

Blunt

Mutagenesis ¹× ±âŸ Á¤È®ÇÑ ÁõÆø»ê¹°À» ÇÊ¿ä·Î ÇÒ °æ¿ì.

 

Sensitivity vs. Stablity for Taq Polymerase

 

 0 month                                          12months

              0.1   0.01  0.001 ng                        0.1    0.01  0.001ng



 

 

 

 

 

 

                                         


PCR products of 16S rRNA fragments from each amount of E. coli genomic DNA

 


VIVATM Taq Polymerase¸¦ ÀÌ¿ëÇÑ °¢ Å©±âº° ÁõÆøÀÇ ¿¹

 

    M     1       2      3     4       5     6    7       8     9    10    11    12    13   14    15    16    M


 

M: Marker, Lane 1~16: 100~1,600 bp Å©±âÀÇ ÁõÆø´ÜÆí.

Lambda DNA¸¦ ÁÖÇüÀ¸·Î PCR ¹ÝÀÀÀ» ¼öÇàÇÏ¿´À½.

 

VIVATM Taq Polymerase¸¦ ÀÌ¿ëÇÑ ÁõÆøÀÇ ¿¹

 

     M         1           2          3


 

 

 

 

 


M: Marker, Lane 1: 5 kbp, Lane 2: 7 kbp, Lane 3: 5+7 kbp

E. coli cololy ÁÖÇüÀ¸·Î PCR ¹ÝÀÀÀ» ¼öÇàÇÏ¿´À½7

 

VIVATM Hot-Taq Polymerase¸¦ ÀÌ¿ëÇÑ ÁõÆøÀÇ ¿¹

 

        M             1           2             3


 

 

 

 

 

 

 

 

 


Calf gDNA¿¡¼­ 980bp DNA fragment ÁõÆø.

M: 100bp ladder Marker

Lane 1,2: VIVATM Taq DNA Polymerase

Lane 3,4: VIVATM Hot-Taq Polymerase

 

 M     1       2         3      4        5       6      7      8





 

 

 

 

 

 

 

 

 

 

 

 


Human gDNA¿¡¼­ 314bp DNA fragment ÁõÆø.

M: 100bp ladder Marker

Lane 1,2: VIVATM Hot-Taq Polymerase

Lane 3,4: Aȍ Hot Start Taq Polymerase

Lane 5,6: VIVATM Taq DNA Polymerase

Lane 7,8: Aȍ Taq DNA Polymerase

 

 

VIVATM Pfu DNA Polymerase¸¦ ÀÌ¿ëÇÑ ÁõÆøÀÇ ¿¹

 

                      MW    1    2       3       4

 



MW: 1kb plus market

Lane 1: 5kb PCR °á°ú¹°, S»ç

Lane 2: 5kb PCR °á°ú¹°, Vivagen»ç

Lane 3: 7kb PCR °á°ú¹°, S»ç

Lane 4: 7kb PCR °á°ú¹°, Vivagen»ç